Cover Picture: To characterize stromal CAFs in the microenvironment of CRC, fresh blood from the patient suffering from metastatic CRC was used for isolating primary CAFs. After 6-7 weeks of incubation, CAFs were seen outgrowing and remained attached to the culture plate. All the unattached cells were removed through washing. The confluent monolayer of CAFs was subjected to trypsinization and expanded by a large amount for further experimentation. Cells were cryopreserved at -85 °C. These cells exhibited higher proliferative potential in vitro and are currently at passage 27.
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