fig4

Figure 4. The influence of the polyphenols in this study and their combinations on the level of γ-H2AX in HL-60 cells after 20 h of incubation. (A) The level of fluorescence intensity of γ-H2AX measured by flow cytometry; (B) representative dot plots of γ-H2AX vs. DNA staining. Curcumin, quercetin and epicatechin did not influence the level of γ-H2AX at the investigated concentrations (A). However the combination of epicatechin (500 μmol/L) and curcumin (40 μmol/L) increased significantly the level of phosphorylated H2AX (A), (B). Values are means ± SEM, *P < 0.05 vs. control, ^#P < 0.05 vs. C40 (ANOVA, Tukey post hoc test). Cur: curcumin; Q: quercetin; EC: epicatechin; RFU: relative fluorescence units